Journal of Indian Society of Periodontology
Journal of Indian Society of Periodontology
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ORIGINAL ARTICLE
Year : 2011  |  Volume : 15  |  Issue : 4  |  Page : 371-375

Effect of a volatile smoke component (acrolein) on human gingival fibroblasts: An in vitro study


1 Department of Periodontics, Sree Balaji Dental College and Hospital, Narayanapuram, Pallikaranai, India
2 Department of Periodontics, Meenakshi Ammal Dental College and Hospital, Maduravoyal, Chennai - 600095, Tamil Nadu, India

Correspondence Address:
Nithya Anand
Department of Periodontics, Sree Balaji Dental College and Hospital, Velachery Main Road, Narayanapuram, Pallikaranai, Chennai - 600 100, Tamil Nadu
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0972-124X.92573

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Aim: Tobacco and some of its volatile and non-volatile components have been found to affect many types of cells including human gingival fibroblasts. The aim of this present study was to estimate the effect of acrolein, a volatile fraction of cigarette smoke on the attachment, proliferation and ultra structure of human gingival fibroblasts in culture. Materials and Methods: Human gingival fibroblasts strains obtained from healthy subjects aged 20-30 years, were grown to confluency and utilized between 3 rd -6 th passages. The cell cultures seeded in 96 well microtitration plates at a density of 45,000 cells/well were incubated with acrolein at concentrations of 10 -4 , 3×10 -5 and 10 -5 . Attachment ability was evaluated after three hours using Neubauer hemocytometer. For the proliferation assay cell cultures seeded at a density of 10,000 cells/well were incubated at concentrations of 10 -4 , 3×10 -5 , 10 -5 , 3×10 -6 , 10 -6 and cell count determined after 5 days using a hemocytometer. Cell morphology was examined under phase contrast microscope. Results: Acrolein produced a dose-dependent cytotoxic effect on human gingival fibroblasts with complete inhibition of attachment and proliferation at higher concentrations. Conclusion: This supports the hypothesis that cigarette smoke is a great risk factor in the development and progression of periodontal disease.


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