Journal of Indian Society of Periodontology
Journal of Indian Society of Periodontology
Home | About JISP | Search | Accepted articles | Online Early | Current Issue | Archives | Instructions | SubmissionSubscribeLogin 
Users Online: 241  Home Print this page Email this page Small font size Default font size Increase font sizeWide layoutNarrow layoutFull screen layout
ORIGINAL ARTICLE
Year : 2014  |  Volume : 18  |  Issue : 2  |  Page : 150-154

mRNA expression of pattern recognition receptors and their signaling mediators in healthy and diseased gingival tissues


1 Shiraz Institute for Cancer Research; Department of Periodontology, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran
2 Department of Periodontology, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran
3 Shiraz Institute for Cancer Research, Shiraz University of Medical Sciences, Shiraz, Iran
4 Department of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
5 Shiraz Institute for Cancer Research, Department of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran

Correspondence Address:
Abbas Ghaderi
Shiraz Institute for Cancer Research, Department of Immunology, School of Medicine, Shiraz University of Medical Sciences, P.O. Box 71345 3119, Shiraz
Iran
Login to access the Email id

Source of Support: This work was supported by a grant from Shiraz Institute for Cancer Research,, Conflict of Interest: None


DOI: 10.4103/0972-124X.131309

Rights and Permissions

Background: Gingivitis and periodontitis are initiated by inflammation caused by microorganisms. Pathogen-associated molecular patterns (PAMPs) from these microorganisms are recognized through various toll-like receptors (TLRs) and NOD-like receptors (NLRs). In this study, we have chosen five TLRs and two NLRs as representatives taking part in the recognition and inflammation process, along with a few of their signaling mediators including CD14, MYD88, and TRIF to compare their mRNA expression levels between healthy and diseased gingival tissues. This will provide deeper insight into the mechanisms underlying gingivitis and periodontitis. Understanding the mechanisms involved in the onset and progression of the periodontal diseases could greatly help in establishing effective ways for prevention and treatment of these diseases besides decreasing the risk factor for relevant systemic disorders. Materials and Methods: Gingival tissue samples for mRNA extraction and cDNA synthesis were taken from patients with gingivitis and periodontitis and from healthy control subjects. Messenger RNA expression of all genes was assessed using real-time polymerase chain reaction (PCR). Results: Among the genes studied in different groups, only MYD88 mRNA expression was significantly higher in the periodontitis group compared to that of the controls. The expression level of this molecule was also significantly higher in patients with severe periodontitis compared to other patients and also compared to healthy individuals. In different tissues, positive significant correlations were observed between the mRNA expression levels of some genes. Conclusions: Elevated mRNA levels of MYD88 in periodontitis might have a key role in the pathogenesis of this disease. Therefore, MYD88 may be a useful target for the therapy of this disease.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed1954    
    Printed31    
    Emailed1    
    PDF Downloaded187    
    Comments [Add]    

Recommend this journal