| ORIGINAL ARTICLE |
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| Year : 2018 | Volume
: 22
| Issue : 6 | Page : 474-479 |
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Evaluation of collagenase-3 matrix metalloproteinase-13 gene-associated polymorphisms 11A/12A and −77A/G and its associated alleles with and without periodontitis
Jammula Surya Prasanna1, Mutyap Divya Aishwarya1, Parupalli Karunakar2, Koduganti Rekharani1, Bodiga Vijayalakshmi3, Puppala Jharna3
1 Department of Periodontics, Panineeya Mahavidyalaya Institute of Dental Sciences and Research Centre, Hyderabad, Telangana, India
2 Department of Conservative and Endodontics, Panineeya Mahavidyalaya Institute of Dental Sciences and Research Centre, Hyderabad, Telangana, India
3 Department of Genetics, Institute of Genetics and Hospital for Genetic Diseases, Hyderabad, Telangana, India
Correspondence Address:
Dr. Jammula Surya Prasanna
Department of Periodontics, Panineeya Mahavidyalaya Institute of Dental Sciences and Research Centre, Kamala Nagar, Road No. 5, Dilsuck Nagar, Hyderabad - 500 060, Telangana
India
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/jisp.jisp_333_18
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Context: Connective tissue devastation in periodontitis and other chronic inflammatory diseases is a major concern. There are several inflammatory mediators associated with this process among which matrix metalloproteinases (MMPs) play a predominant role. Collagen degradation is primarily mediated by the collagenases. MMP-13 is familiar as collagenase-3, which has the aptitude to humiliate fibrillar collagen. Aims: This study aims to evaluate MMP-13 promoter polymorphism, 11A/12A, and −77A/G and associated alleles in patients with and without chronic periodontitis (CP). Settings and Design: This was an observational case–control study. Materials and Methods: Of the total 100 patients, 50 with CP (test group) and 50 without CP (Control group), blood was collected for deoxyribonucleic acid isolation. The 11A/12A and −77A/G polymorphisms of the MMP-13 gene were picked out by polymerase chain reaction (PCR)- single-strand conformation polymorphism analysis method and PCR-restriction fragment length polymorphism by BseNI restriction enzyme, respectively. Statistical Analysis Used: Association between MMP-13 genotype (GTs) (11A/12A, 11A/11A, 12A/12A) and (AA, AG, GG) was assessed by Chi-square and Student's t-test for intergroup comparison. Results: 11A/12A GT was seen in 24% and 20%, 11A/11A 64% and 72%, 12A/12A 12% and 8% in test and control groups, respectively. However, the association was not statistically significant. −77A/G polymorphism associated GT s AA was 56% and 62%, AG 24% and 28%, GG 20% and 10% in test and controls, respectively. An association of GG was statistically significant. Conclusion: The present study results indicated that MMP-13 −77A/G gene polymorphisms, GG GT may be predicted intensive ability for CP. On the other hand, there was no significant association between MMP-13 11A/12A gene polymorphisms with CP.
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