Journal of Indian Society of Periodontology
Journal of Indian Society of Periodontology
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ORIGINAL ARTICLE
Year : 2021  |  Volume : 25  |  Issue : 1  |  Page : 11-16

Effect of epigallocatechin-3-gallate on tumor necrosis factor-alpha production by human gingival fibroblasts stimulated with bacterial lipopolysaccharide: An in vitro study


1 Department of Periodontics, School of Dentistry, Shahid Sadughi University of Medical Science, Yazd, Iran
2 Department of Periodontics, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3 Department of Immunology; Blood and oncology research center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
4 Department of Periodontics, School of Dentistry, Shahid Sadughi University of Medical Sciences, Yazd, Iran
5 Department of Immunology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

Correspondence Address:
Elahe Karami
Department of Periodontics, School of Dentistry, Shahid Sadughi University of Medical Science, Yazd
Iran
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/jisp.jisp_323_20

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Background: Evidence shows that epigallocatechin-3-gallate (EGCG) in green tea has anti-inflammatory effects. Aim: This study assessed the effect of EGCG on the production of tumor necrosis factor-alpha (TNF-α) as an inflammatory cytokine in periodontitis, which produced by human gingival fibroblasts (HGFs) stimulated with lipopolysaccharide (LPS) of Porphyromonas gingivalis. Materials and Methods: In this study, HGFs were cultured and subjected to LPS and EGCG. Cell viability of different concentrations of EGCG (10, 25, 50, 75, and 100 μM) and LPS (1, 10, 20, and 50 μg/mL) was assessed using methyl-thiazole-tetrazolium (MTT) assay. Then, the best concentrations of EGCG and P. gingivalis LPS were used simultaneously and separately to assess the production of TNF-α by HGFs using the enzyme-linked immunosorbent assay (ELISA). Assessments were done at 1, 3, and 5 days. Data were read using the ELISA reader and analyzed by the SPSS through two-way ANOVA. Results: LPS at 1, 10, and 20 and EGCG at 10.25 and 50 μM showed the least cytotoxicity in MTT assay. ELISA showed EGCG alone decreased the production of TNF-α in all days, except 10 μM on day 1. 1, 10, and 20 μg/mL LPS increased the output of TNF-α on days 1 and 3 while reducing it on day 5. The combination of EGCG and LPS showed a decrease of TNF-α in all days except on day 5 that revealed an increase in the production of TNF-α at 25 and 50 μM EGCG. Conclusion: In the combination use of EGCG and LPS, EGCG shows anti-inflammatory effects by decreasing the production of TNF-α by HGFs stimulated with P. gingivalis.


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