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Year : 2022  |  Volume : 26  |  Issue : 1  |  Page : 19-23  

Evaluation of mechanical properties of platelet-rich fibrin membrane for implant surgery: An analysis in vitro

1 Department of Dentistry, Ingá University Center, Maringá, Brazil
2 Department of Diagnosis and Surgery, São Paulo State University, Araraquara, São Paulo, Brazil
3 Department of Nucleus of Applied Research in Morphology and Immunology, Faculty of Medicine, University of Brasília, Brasília, Brazil
4 Department of Science and Technology, Chapecó, Santa Catarina, Brazil

Date of Submission18-Nov-2020
Date of Decision08-May-2021
Date of Acceptance24-Jun-2021
Date of Web Publication01-Jan-2022

Correspondence Address:
Ísis de Fátima Balderrama
Department of Diagnosis and Surgery, Araraquara School of Dentistry, Sao Paulo State University, St. Humaita, 1680, 14801 385, Araraquara, SP
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/jisp.jisp_782_20

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The aim of this study was to evaluate and compare the mechanical resistance of platelet-rich fibrin (PRF) membrane when submitted to resistance traction on longitudinal axis. Blood collection of a healthy individual was collected with an amount of 300 mL and divided into 30 tubes containing 10 mL each one. The samples were divided into three groups, according to the g-force protocols: (1) F200 g: (2) F400 g; (3) F800 g. Membranes of each g-force group were divided into subgroups, according to waiting time after centrifugation: (T0) immediate use and (T30) use after 30 min. Considering these intervals of time, the concentrate was removed from tubes and inserted in a PRF metallic box to confection of the membrane. The PRF membranes were submitted to mechanical tension on a universal testing machine and obtained to a resistance force of each membrane. The centrifugation time showed no statistically significant difference for membrane resistance for any force applied (P > 0.05; Student's t-test). For T0 group results demonstrated no influence for membrane resistance (P = 0.357; Student's t-test), therefore T30 showed statistically significant difference (P = 0.040; Student's t-test) for membrane resistance for centrifugation forces applied, with highest value when applied greatest force. The findings suggest that the waiting time for centrifugation could be determined according to demand of application, and for immediate use of the membrane, the centrifugation did not influence the resistance, on the other hand, after 30 min, the application of higher force resulted in a membrane with considerable resistance.

Keywords: Centrifugation, dental implants, platelet-rich fibrin, protocols, resistance traction

How to cite this article:
Petronilho VG, Balderrama &d, de Oliveira LA, Queiroz PM, Zubek MG, Gottardo VD. Evaluation of mechanical properties of platelet-rich fibrin membrane for implant surgery: An analysis in vitro. J Indian Soc Periodontol 2022;26:19-23

How to cite this URL:
Petronilho VG, Balderrama &d, de Oliveira LA, Queiroz PM, Zubek MG, Gottardo VD. Evaluation of mechanical properties of platelet-rich fibrin membrane for implant surgery: An analysis in vitro. J Indian Soc Periodontol [serial online] 2022 [cited 2022 Jan 19];26:19-23. Available from:

   Introduction Top

The oral rehabilitation with dental implants can find problems due when appears bone resorption with lack of hard tissue around implants, demonstrating failures for support of osseointegrated implants.[1],[2]

Several approaches through regenerative procedures with biomaterials can be used for bone and tissue regeneration, such as, synthetic membrane, grafts, bioactive growth factors, and enamel matrix derivatives are some materials that can be used for regeneration.[3] In addition, the use of autologous blood concentrated is an alternative to be considered.[4]

The membrane leukocytes platelet-rich fibrin (L-PRF) in polymerized and gelled form and has function of a three-dimensional framework. This concentrate is composed of platelets, leukocytes, platelet micro particles, glycoprotein, and several plasma proteins.[5] In addition, these concentrates has function to release growth factors such as, growth factor beta, platelet-derived growth factor and endothelial growth factor. The presence of these elements can benefit the healing process, angiogenesis and bone neo-formation in the surgical area to obtain bone regeneration.[6],[7]

The concentrate is obtained after human blood centrifugation, without additives and this process can be performed through different forces and times; the literature demonstrated a wide variety of possibilities regarding forces and times for centrifugation.[8] The centrifugation processes can influence the characteristics of the membrane.[9],[10] Ghanaati et al.[9] demonstrated that when membrane was harvested from the lowest centrifugation force showed as a porous fibrin and with density lower, however, a greater number of leukocytes and growth factors were found added on the clot.

Considering the variety of centrifugation protocols and their influence on the characteristics of the membranes obtained, the literature demonstrates that is necessary to evaluate the centrifugation protocol which can influence directly in the resistance of membrane according to traction.[8],[9],[10]

Otherwise, considering the clinical application, to obtain the membrane can be performed at the beginning of the surgical procedure or immediately before the use, it means, during the surgical procedure, interfering in the fibrin polymerization time.

The aim of this study was to evaluate the resistance of the membranes of PRF through different centrifugation protocols and intervals between application and use of membrane.

   Materials and Methods Top

This study was performed in line with the principles of the Declaration of Helsinki and approved by Human Research Ethics Committee under protocol number 3.552.132 (Uningá University, Maringá, Paraná, Brasil) and the patient signed an informed consent agreement.

To obtain the samples of this study were collected 300 mL of blood from a patient donator. The volunteer was a male with 34 years old, leukoderma and systemically healthy. In laboratory analysis, the sample obtained showed blood count and coagulogram values within the reference standard. The volume total was divided into 30 tubes, each one containing 10 mL.

The samples were divided into three groups, according to the g-force (F) applied during centrifugation:

  1. Group 1: F200 m/s2
  2. Group 2: F400 m/s2
  3. Group 3: F800 m/s2.

[Figure 1] shows the centrifuge acquired for this study, for centrifugations during 10 min of the all samples through centrifuge FibrinFuge® (Monteserrat, Changzhou Jiangsu, China). The physical characteristics of the membranes, independent of the applied force, were opaque yellow coloration in 2/3 from extension and red coloration in 1/3 from membrane. The membrane was 50 mm long and 2 mm thick, with a smooth texture and gelatinous consistency. Each group was subdivided into two situations: Immediate preparation of the membrane (T0) or preparation of the membrane with 30 min after centrifugation (T30). Independent of the waiting time, for membrane confection the blood concentrates were maintained into the metallic box for the time of 2 min and 30 s for pressing.
Figure 1: Centrifuge obtained to perform the processing of samples with different forces evaluated (FibrinFUGE 25, Montserrat®)

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After making the membrane, immediately they were fixed by on a paper support [Figure 2]. An acrylic claw device [Figure 3] was developed to fixation of sample-support conjunct. The device was designed and cut in a universal testing machine with LASER SL1390 (Suda, Heifi, China). The sample-support conjunct was fixing into this device and coupled in a universal testing machine (Instron 5967, Canton, Massachusetts EUA) to perform the mechanical analysis.
Figure 2: Paper support developed to evaluate the membrane attached into longitudinal axis

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Figure 3: Device developed to fix the sample-support on the universal testing machine

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A 50N loading was applied to obtain g-force on the long vertical axis of the membrane. The traction force was applied until the total rupture of the membrane [Figure 4]a,[Figure 4]b,[Figure 4]c. After the disruption, the Bluehill 3 software (Canton, Massachusetts, USA) that was coupled in a universal testing machine system, registered the force necessary to initiate the rupture of the membrane.
Figure 4: (a) Membrane with traction; (b) Zoom of membrane with traction; (c) Membrane after rupture due the traction force applied

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Scanning electron microscope images (JEOL JSM-7610F) were analyzed after rupture of membranes magnification × 50, ×1000 and ×2500 [Figure 5] and [Figure 6].
Figure 5: Leukocytes platelet-rich fibrin membrane after rupture with high magnification by Scanning Electron Microscope. (a) ×50; (b) ×1000; (c) ×2500 *Note the fibrin network

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Figure 6: Leukocytes platelet-rich fibrin membrane after rupture (specifically the buffy coat area) with high magnification by Scanning Electron Microscope. (a) ×50; (b) ×1000; (c) ×2500

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The data were collected and submitted for statistical analysis (IBM SPSS Statistic Software, Chicago, USA). To comparisons between resistance force (RF) of membranes obtained within different times were performed Student's t-test of independent sample; and to comparison of different forces, was performed ANOVA with post hoc Tukey's, when applicable. The significance level of 5% was considered.

   Results Top

[Table 1] shows the value of the RF required to break up the membrane considering the differences on applied force and waiting time evaluated. No significant difference was observed regarding to the RF of the membrane used in the different studied times (T0 and T30), for any force applied in the centrifugation, such as, F200, F400 and F800 (P = 0.2169, P = 0.8849 and P = 0.1172; Student's t test).
Table 1: The values of the resistance force groups (mean±standard deviation)

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There was no significant difference in the membranes evaluated immediately after centrifugation, regardless of the force applied in the centrifugation process (P = 0.3750; Student's t-test).

On the other hand, after waiting time (30 min) for using the membrane, showed a significant increase in resistance (P = 0.040; Student's t-test) between F200 and F800 groups. Therefore, the membrane from F400 group showed no difference in resistance when compared to F200 (P = 0.762; Student's t test) and F800 (P = 0.317; student's t-test) with waiting time of 30 min.

   Discussion Top

The development of method of obtaining the blood concentrates by Choukroun et al.,[11] showed the evolution in search to increase the cell concentration and consequently the regenerative potential of this fibrin matrix. It was analyzed that when the centrifugation force is decreased, a blood concentrate is most porous and cellular and less dense.[9],[12]

When the autologous blood is collected to membrane confection, no additives are incorporated into the material.[13],[14] The g-force is able to perform the segmentation of the blood layers, making it possible to obtain the membrane.[15] This procedure is relatively simple, can be performed at the clinic practice and has some advantages for bone grafts.[15],[16],[17]

The use of PRF can be applied with different clinical fields, implant dentistry, surgery, and periodontology.[18] Otherwise, is known that the time and force performed in the centrifugation can influence the characteristics of the membrane that will be applied in the surgical receptor area.[8],[19]

Membranes obtained from concentrates that are prepared with centrifugation force. It is calculated considering the size of the radius from centrifuge and the rotations per minute. A lesser centrifugation force shows greater release of growth factors and less degradation of fibrin.[19] In addition, lower centrifugation force results in a membrane with greater regeneration potential.[19],[20],[21],[22] The biochemical characteristics influence the final composition of membrane to surgical approach or for another reason; the fact to increase membrane density is observed with centrifugation force[9] confirms to questions about the resistance of material. Therefore, this present was performed to aim to analyze the mechanical resistance of the membrane when subjected to traction (mN) on long axis according to both extremities until their rupture.

Although of preparation membrane process is considered a simple and easy procedure, some aspects should be considered.[14],[15] For surgical procedures, the membrane can be used during clinic practice, otherwise, the blood centrifuged can be prepared before begin the surgery or during the surgical procedure. In our study, we could observed that the waiting time did no showed influence regarding resistance membrane. Thus, the blood collect could be performed in the moment most convenient for the patient and professional.

On the other hand, when is stabilized that the waiting time to the preparation of membrane after centrifugation, it can be necessary to consider the force applied. In our results was observed that membranes obtained immediately after centrifugation showed no influence regarding resistance in function to applied force.

On the other hand, through the waiting time group analysis, the results showed a greater resistance of membrane when the centrifugation force was applied. It can suggest that when applied the waiting time with 30 min, the polymerization process begins with an acceleration format into membranes obtained with a major force, therefore, over time the influence of force in relation to resistance becomes remarkable. This membrane property with a major force of centrifugation shows higher density and less porosity[8],[9] and it does not necessarily reflect in their resistance. Thus, for centrifugation with less force it can consider the presence of higher concentration of cellular components and growth factors,[9] whenever possible, immediate application provides to be a good viable alternative for procedures.

That protocol of centrifugation with less force will provide a greater concentration of membrane components without compromising the material resistance. However, more studies are necessary to prove this information and to evaluate other possible factors that may influence the resistance properties. Otherwise, clinical studies are essential to evaluate the impact of different resistances with waiting time for 30 min.

   Conclusion Top

Within the limitation of this in vitro study, it can conclude that among the times evaluated, the collection and material centrifugation can be performed at the most appropriate time for the patient and professional. However, when there is a waiting time of 30 min after centrifugation, the applied force must be greater to increase the resistance of the membrane.

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Conflicts of interest

There are no conflicts of interest.

   References Top

Anitua E. Plasma rich in growth factors: Preliminary results of use in the preparation of future sites for implants. Int J Oral Maxillofac Implants 1999;14:529-35.  Back to cited text no. 1
Sculean A, Gruber R, Bosshardt DD. Soft tissue wound healing around teeth and dental implants. J Clin Periodontol 2014;41 Suppl 15:S6-22.  Back to cited text no. 2
Hollander A, Macchiarini P, Gordijn B, Birchall M. The first stem cell-based tissue-engineered organ replacement: Implications for regenerative medicine and society. Regen Med 2009;4:147-8.  Back to cited text no. 3
Miron RJ, Zucchelli G, Pikos MA, Salama M, Lee S, Guillemette V, et al. Use of platelet-rich fibrin in regenerative dentistry: A systematic review. Clin Oral Investig 2017;21:1913-27.  Back to cited text no. 4
Dohan DM, Choukroun J, Diss A, Dohan SL, Dohan AJ, Mouhyi J, et al. Platelet-rich fibrin (PRF): A second-generation platelet concentrate. Part I: technological concepts and evolution. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006;101:e37-44.  Back to cited text no. 5
Andia I, Abate M. Platelet-rich plasma: Underlying biology and clinical correlates. Regen Med 2013;8:645-58.  Back to cited text no. 6
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Ghanaati S, Booms P, Orlowska A, Kubesch A, Lorenz J, Rutkowski J, et al. Advanced platelet-rich fibrin: A new concept for cell-based tissue engineering by means of inflammatory cells. J Oral Implantol 2014;40:679-89.  Back to cited text no. 9
El Bagdadi K, Kubesch A, Yu X, Al-Maawi S, Orlowska A, Dias A, et al. Reduction of relative centrifugal forces increases growth factor release within solid platelet-rich-fibrin (PRF)-based matrices: A proof of concept of LSCC (low speed centrifugation concept). Eur J Trauma Emerg Surg 2019;45:467-79.  Back to cited text no. 10
Choukroun J, Adda F, Schoeffer C, Vervelle A. PRF: An opportunity in perio-implantology (in French). Implantodontie. 2000;42:55-62.  Back to cited text no. 11
Marx RE. Platelet-rich plasma: Evidence to support its use. J Oral Maxillofac Surg 2004;62:489-96.  Back to cited text no. 12
Toffler M, Toscano N, Holtzclaw D, Corso MD, Dohan Ehrenfest DM. Introducing Choukroun's platelet rich fibrin (PRF) to the reconstructive surgery milieu. J Implant Adv Clin Dent 2009;1:21-30.  Back to cited text no. 13
Sezgin Y, Uraz A, Taner IL, Çulhaoğlu R. Effects of platelet-rich fibrin on healing of intra-bony defects treated with anorganic bovine bone mineral. Braz Oral Res 2017;31:e15.  Back to cited text no. 14
Feigin K, Shope B. Use of platelet-rich plasma and platelet-rich fibrin in dentistry and oral surgery: Introduction and review of the literature. J Vet Dent 2019;36:109-23.  Back to cited text no. 15
Marx RE, Carlson ER, Eichstaedt RM, Schimmele SR, Strauss JE, Georgeff KR. Platelet-rich plasma: Growth factor enhancement for bone grafts. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1998;85:638-46.  Back to cited text no. 16
Whitman DH, Berry RL, Green DM. Platelet gel: An autologous alternative to fibrin glue with applications in oral and maxillofacial surgery. J Oral Maxillofac Surg 1997;55:1294-9.  Back to cited text no. 17
Strauss FJ, Nasirzade J, Kargarpoor Z, Stähli A, Gruber R. Effect of platelet-rich fibrin on cell proliferation, migration, differentiation, inflammation, and osteoclastogenesis: A systematic review of in vitro studies. Clin Oral Investig 2020;24:569-84.  Back to cited text no. 18
de Oliveira LA, Borges TK, Soares RO, Buzzi M, Kückelhaus SA. Methodological variations affect the release of VEGF in vitro and fibrinolysis' time from platelet concentrates. PLoS One 2020;15:e0240134.  Back to cited text no. 19
Choukroun J, Ghanaati S. Reduction of relative centrifugation force within injectable platelet-rich-fibrin (PRF) concentrates advances patients' own inflammatory cells, platelets and growth factors: The first introduction to the low speed centrifugation concept. Eur J Trauma Emerg Surg 2018;44:87-95.  Back to cited text no. 20
Kubesch A, Barbeck M, Al-Maawi S, Orlowska A, Booms PF, Sader RA, et al. A low-speed centrifugation concept leads to cell accumulation and vascularization of solid platelet-rich fibrin: An experimental study in vivo. Platelets 2019;30:329-40.  Back to cited text no. 21
Miron RJ, Pinto NR, Quirynen M, Ghanaati S. Standardization of relative centrifugal forces in studies related to platelet-rich fibrin. J Periodontol 2019;90:817-20.  Back to cited text no. 22


  [Figure 1], [Figure 2], [Figure 3], [Figure 4], [Figure 5], [Figure 6]

  [Table 1]


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