Journal of Indian Society of Periodontology

ORIGINAL ARTICLE
Year
: 2011  |  Volume : 15  |  Issue : 4  |  Page : 366--370

Analysis of association between TLR-4 Asp299Gly and Thr399Ile gene polymorphisms and chronic periodontitis in a sample of south Indian population


Bavigadda Harish Reddy1, ND Jayakumar2, Sreenivasa Rao Akula1, Rupali Sharma2, G Kaarthikeyan2, Sankari2 
1 Department of Periodontics, SVS Institute of Dental Sciences, Appannapally, Mahabubnagar, Andhra Pradesh, India
2 Department of Periodontics, Saveetha Dental College and Hospital, Chennai, Tamil Nadu, India

Correspondence Address:
Bavigadda Harish Reddy
Department of Periodontics, SVS Institute of Dental Sciences, Appannapally, Mahabubnagar - 509 002, Andhra Pradesh
India

Background: To analyze the association between TLR-4 Asp299Gly and Thr399Ile gene polymorphisms and chronic periodontitis in a sample of south Indian population. Materials and Methods: Genomic DNA was obtained from peripheral blood of 60 patients with chronic periodontitis and 60 periodontally healthy subjects. TLR-4 Asp299Gly and Thr399Ile gene polymorphisms were genotyped by a polymerase chain reaction-restriction fragment length polymorphism method. The data were analyzed by a χ2-test and by relative risk estimation. Results: Thr399Ile alleles were found in 4% of chronic periodontitis patients and in 1% of periodontally healthy subjects. The prevalence of a Thr399Ile heterozygote was found to be 5% in the chronic periodontitis group and 1.67% in the periodontally healthy group, respectively. Homozygosity for TLR-4 Thr399Ile was seen in chronic periodontitis patients only, which was 1.67%. The TLR-4 Asp299Gly gene polymorphism was not detected in either chronic periodontitis or periodontally healthy groups. Conclusion: There is no significant association between TLR-4 Thr399Ile polymorphism and chronic periodontitis in a sample of south Indian population.


How to cite this article:
Reddy BH, Jayakumar N D, Akula SR, Sharma R, Kaarthikeyan G, Sankari. Analysis of association between TLR-4 Asp299Gly and Thr399Ile gene polymorphisms and chronic periodontitis in a sample of south Indian population.J Indian Soc Periodontol 2011;15:366-370


How to cite this URL:
Reddy BH, Jayakumar N D, Akula SR, Sharma R, Kaarthikeyan G, Sankari. Analysis of association between TLR-4 Asp299Gly and Thr399Ile gene polymorphisms and chronic periodontitis in a sample of south Indian population. J Indian Soc Periodontol [serial online] 2011 [cited 2021 May 14 ];15:366-370
Available from: https://www.jisponline.com/article.asp?issn=0972-124X;year=2011;volume=15;issue=4;spage=366;epage=370;aulast=Reddy;type=0